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Chromatography

UHPLC 1521 Pro

UHPLC with ultra-high pressure till 22000 psi
feature

UHPLC

Pressure up to 22000 psi

Flexible choices on injection mode

Multiple selection of functions

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UHPLC 1521 Pro

UHPLC 1521 Pro

High-Pressure UHPLC for Fast, Efficient Separations

A purpose-built UHPLC platform designed for high-pressure separations, flexible injection strategies and demanding analytical workflows.

Key Capabilities

Parameter

Specification

System Type

UHPLC

Pump

Up to 18,000 psi / 124 MPa

Flow Range

Up to 5.000 mL/min for UHPLC configuration

Injection Modes

Full Loop / Partial Loop / μL Pick-Up / FTN

Sample Capacity

Up to 216 × 1.5 mL vials

Carryover

<0.0025% under specified conditions

Column Oven

Up to 90°C

Cooling

Down to 4°C

Detector Options

UV-Vis / DAD / FLD / RID and other compatible detectors

 


 

Product Overview

The BIKAI 1521 Pro is a dedicated UHPLC platform developed for laboratories requiring higher pressure capability, faster separations and flexible sample handling.

Its high-pressure pump architecture supports modern UHPLC columns and high-efficiency chromatographic methods, while configurable autosampling and temperature-control options allow the system to adapt to different method and throughput requirements.

The modular detector architecture also enables the system to be configured according to the analytical characteristics of the target compounds.

 


 

UHPLC Pump

The 1521 Pro can be configured with next-generation UHPLC pump options according to required pressure capability.

Parameter

 

Maximum Pressure

18,000 psi

Pressure Equivalent

124 MPa

Application

UHPLC

Pump Features

· High-pressure binary gradient configuration

· Flow range up to 5.000 mL/min

· Flow increment: 0.001 mL/min

· Online degassing

· Designed for high-pressure UHPLC separations

· Pressure pulsation control for stable chromatographic operation

 


 

Autosampler

The 1521 Pro supports multiple injection strategies for both conventional and high-throughput UHPLC workflows.

Parameter

Specification

Injection Modes

Full Loop / Partial Loop / μL Pick-Up / FTN

Loop Options

10 / 20 / 50 / 100 / 1000 / 5000 μL

Standard Capacity

108 × 1.5 mL vials

High-Throughput Capacity

216 × 1.5 mL vials

Microplate Capacity

Up to 4 × 96-well plates

Preparative Configuration

80 × 5 mL vials

Carryover

<0.0025% under specified conditions

Cooling

Optional

Injection Repeatability

Injection Mode

Repeatability

Full Loop

≤0.3% RSD

Partial Loop

≤0.3% RSD under specified volume conditions

μL Pick-Up

≤1.0% RSD under specified conditions

 


 

Column Oven

Parameter

Specification

Temperature Control

Forced-air circulation / Peltier

Heating Range

Ambient +5°C to 90°C

Cooling Range

Down to ambient −21°C

Minimum Temperature

4°C

Temperature Accuracy

±0.5°C

Temperature Stability

±0.1°C

Column Capacity

Up to 4 × 300 mm columns

Optional Column Management

· Automatic switching valves

· PEEK switching valves

· Multiple two-position six-port valve configurations

· Two-position ten-port valve options

· Parallel switching of multiple columns

· Configurations for SPE and multidimensional chromatography

 


 

Choose Your Detector

The 1521 Pro can be configured with different detector technologies according to the analytical requirement.

UV-Vis

Parameter

Specification

Wavelength Range

190–900 nm

Light Source

Deuterium + Tungsten lamps

Wavelength Accuracy

±0.5 nm

Wavelength Precision

±0.1 nm

 


 

DAD

Parameter

Specification

Wavelength Options

190–640 nm / 190–800 nm

Array

1024 pixels

Spectral Resolution

0.6 nm/pixel

Wavelength Accuracy

±0.6 nm

Wavelength Precision

±0.1 nm

Standard Flow Cell

12 μL, 10 mm optical path

Low-Volume Option

2 μL, 10 mm optical path

High-Sensitivity Option

12 μL, 60 mm optical path

 


 

FLD

Parameter

Specification

Excitation Range

200–750 nm or 200–900 nm

Emission Range

200–750 nm or 200–900 nm

Light Source

Xenon Lamp

Water Raman S/N

1200:1

Sampling Rate

100 Hz

Flow Cell

12 μL

 


 

RID

Parameter

Specification

Refractive Index Range

1–1.75

Baseline Noise

<2.5 nRIU

Baseline Drift

<200 nRIU/h

Flow Cell Volume

8 μL

Maximum Flow Rate

10 mL/min

Temperature Control

30–60°C

Temperature Accuracy

±0.1°C

 


 

Software

The BIKAI ChromCore chromatography workstation provides integrated instrument control, acquisition, processing and reporting.

Function

Capability

Instrument Control

Pump, autosampler, oven and detector control

Data Acquisition

Integrated chromatographic acquisition

Data Processing

Automatic integration and data-processing algorithms

Audit Trail

Traceable operation records

User Management

Multi-level permissions

Electronic Signature

Supported

Regulatory Support

FDA 21 CFR Part 11 / GMP / GLP

Reporting

Standard and customizable reports

Maintenance Management

Consumable-life and maintenance reminders

 

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